Thermo Fisher Scientific CD22 Monoclonal Antibody (IS7), APC

Applications

• Flow Cytometry (Flow): 10 µL / 1×10⁶ cells

Product Specifications

Additional Formats

• Unconjugated (MA1-10141)
• FITC (MA1-10144)
• PE (MA1-10145)
• Custom conjugation available

Product Specific Information

This antibody reacts with an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes. It is lost on plasma cells, peripheral blood T lymphocytes, granulocytes, and monocytes.

Target Information

CD22 (BL-CAM) is a type I integral membrane glycoprotein (130–140 kDa) expressed in both the cytoplasm and membrane of B-lymphocytes. CD22 appears early in B-cell differentiation, similar to CD19. Unlike other B-cell markers, CD22 membrane expression is limited to mature B cells (CD22⁺) and lost in plasma cells (CD22⁻), making it useful in phenotyping mature leukemias. CD22 is strongly expressed in hairy cell leukemia and is also positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.

CD22 binds preferentially to α2,6-linked sialic acid; this recognition site can be masked by cis interactions on the same cell surface. Ligand-induced tyrosine phosphorylation during immune response suggests a regulatory role in B-cell antigen receptor signaling. CD22 may act as both a positive regulator via interaction with Src family tyrosine kinases and as an inhibitory receptor by recruiting cytoplasmic phosphatases that block signal transduction through dephosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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