Thermo Fisher Scientific H3.3 G34W oncohistone mutant Recombinant Rabbit Monoclonal Antibody (4H32L4), ChIP-Verified
H3.3 G34W oncohistone 변이체를 특이적으로 인식하는 재조합 토끼 단클론 항체입니다. Western blot, ICC/IF, ChIP assay에 최적화되어 있으며, ChIP 검증 완료 제품입니다. Human에 반응하며 Mouse, Rat, Bovine에도 예측 반응합니다. Protein A 정제, PBS buffer, 0.5 mg/mL 농도.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | 1–5 µg/million cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4H32L4 |
| Immunogen | Peptide corresponding to human H3.3G34W [aa30–aa39] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2895884 |
Product Specific Information
This antibody is predicted to react with Mouse, Rat, and Bovine.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher-order chromatin structures.
This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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