Thermo Fisher Scientific H3K27me1 Recombinant Rabbit Monoclonal Antibody (28H59L67), ChIP-Verified
H3K27me1 단일클론 항체로 ChIP, WB, ICC/IF 등 다양한 응용에 적합. 인간 및 생쥐 반응성. Expi293 발현 시스템에서 제작된 recombinant rabbit IgG. Protein A로 정제된 액상 제품으로 안정적인 저장 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | 1–5 µg/million cells |
| Peptide Array (Array) | 1:2000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 28H59L67 |
| Immunogen | Peptide corresponding to human H3K27me1 [aa23–aa31] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2895882 |
Product Specific Information
This antibody is predicted to react with Rat and Bovine.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA to form the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones can change chromatin structure or facilitate binding of specific transcription factors. The N-terminal tail of histone H3 can undergo various modifications such as methylation, acetylation, or phosphorylation, influencing cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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