Thermo Fisher Scientific LCK Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Positive Control: Molt-4
• Predicted Reactivity: Mouse (96%), Rat (96%), Pig (95%), Sheep (95%), Bovine (95%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

LCK is a member of the Src-family tyrosine kinases, essential for signaling through the T-cell receptor (TCR) complex. Upon TCR activation, LCK phosphorylates ITAM motifs in the zeta subunits, enabling binding of ZAP70, which is subsequently phosphorylated and activated by LCK. This cascade leads to downstream signaling via adaptor LAT.

LCK localizes mainly to the plasma membrane but is also associated with the Golgi apparatus, potentially contributing to Raf activation under weak TCR stimulation. The protein contains N-terminal myristylation and palmitylation sites, a PTK domain, and SH2/SH3 domains mediating protein-protein interactions. LCK also regulates apoptosis induced by various stimuli, excluding death receptor pathways. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternative splice variants produce different isoforms of the protein.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.