Thermo Fisher Scientific PAPD5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PAPD5. Recombinant protein control fragment (Product #RP-98642).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2645197

Product Specific Information

Immunogen sequence: YYPNNETESI LGRIIRVTDE VATYRDWISK QWGLKNRPEP SCNGNGVTLI VDTQQLDKCN NNLSEENEAL GKCRSKTSES LSKHSSNSSS GPVS

Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 94%.

Target Information

Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3poly(A) tail creating a heterogeneous 3 poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation (PubMed:21788334, PubMed:30026317). Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesnt need a cofactor for polyadenylation activity (in vitro) (PubMed:21788334, PubMed:21855801). Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1 (PubMed:28383716). Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion (PubMed:18172165). Mediates 3 adenylation of the microRNA MIR21 followed by its 3-to-5 trimming by the exoribonuclease PARN leading to degradation (PubMed:25049417). Mediates 3adenylation of H/ACA box snoRNAs (small nucleolar RNAs) followed by its 3-to-5` trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation (PubMed:22442037). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.