Thermo Fisher Scientific CD62E/CD62P Monoclonal Antibody (1.2B6)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

1-10 µg/mL

-

Flow Cytometry (Flow)

10 µg/mL

-

ELISA (ELISA)

10 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Inhibition Assays (IA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Pig

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1.2B6

Immunogen

Human E-Selectin (ELAM-1).

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_929204

Product Specific Information

MA1-80430 inhibits neutrophil adhesion to E-selectin and to P-selectin.

Non-reducing conditions are recommended for Western blot applications. A suggested positive control is human tonsil. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 recognizes the human CD62E and CD62P cell surface antigens.

Target Information

CD62E represents the 97 kDa type I transmembrane glycoprotein E-selectin, also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1). It is expressed on cytokine-stimulated endothelium, weakly on unactivated endothelium, and is absent on leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial attachment and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues.CD62P is a 140 kDa membrane glycoprotein formerly known as platelet activation-dependent granule membrane protein (PADGEM) or GMP-140. It is expressed on platelets, megakaryocytes, and epithelium. It is thought to mediate the adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses and of activated platelets to neutrophils and monocytes in hemostasis. The monoclonal antibody 1.2B6 recognizes a common epitope shared by CD62E and CD62P.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.