Thermo Fisher Scientific T-Lymphocytes Monoclonal Antibody (KEN-5), FITC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Neat

-

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

KEN-5

Immunogen

Rabbit thymocytes.

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_935568

Product Specific Information

This clone recognizes rabbit (lapine) T-cells, including >90% thymocytes and approximately 40-45% mesenteric lymph node cells. It immunoprecipitates a 67 kDa protein from rabbit thymocytes. This antibody does not bind to rabbit CD5 transfectants and shows very limited binding to rabbit B-cells, which in adult rabbits have been demonstrated to express CD5. This clone may recognize an epitope that is dependant on glycosylation to maintain its structural configuration, which may explain why the antibody does not recognize recombinant rabbit CD5 produced in insect cells (which contain different glycans from those found in mammalian cells).

For flow cytometry, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

T Lymphocytes are a subtype of white blood cell that is central to cell-mediated immunity. T Lymphocytes mature in the thymus from thymocytes. The majority of human T cells rearrange their alpha and beta chains on the cell receptor and are termed alpha beta T cells (alpha/beta T cells) and are part of the adaptive immune system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.