Thermo Fisher Scientific Bacteriophage M13 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

1:10,000-1:50,000

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-M13 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of Bacteriophage M13 conjugated to Keyhole Limpet Hemocyanin (KLH).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against Tail virion protein G7P. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence with Enterobacteria phage M13, Enterobacteria phage f1, and Enterobacteria phage fd.

Target Information

Tail virion protein G7P (Bacteriophage M13) may initiate with G9P the virion concomitant assembly-budding process, by interacting with the packaging signal of the viral genome. The assembly-budding takes place at the host inner membrane. In turn, G7P and G9P are present at the end of the filamentous virion that emerges first from the bacterial host. Anti-Bacteriophage M13 Antibody is useful for researchers interested in HCP, Host Cell Membranes, Transmembrane Viruses, and E. coli bacteria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.