Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), eBioscience
인간 IL-10을 인식하는 JES3-9D7 클론의 단클론 항체. ELISA, Flow Cytometry, Neutralization 등 다양한 어플리케이션에 사용 가능. 0.5 mg/mL 농도의 액상 형태로 제공되며, Affinity chromatography로 정제됨. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1000 | - |
| Immunohistochemistry (IHC) | - | 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Flow Cytometry (Flow) | - | 18 publications |
| ELISA | 1–4 µg/mL | 8 publications |
| Neutralization (Neu) | - | 2 publications |
| Functional Assay | - | 1 publication |
| Miscellaneous PubMed | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Cynomolgus monkey, Human, Mouse, Non-human primate |
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JES3-9D7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468438 |
Additional Available Formats
- Alexa Fluor 488 (#53-7108-42)
- Brilliant UV 395 (#363-7108-42)
- Brilliant UV 737 (#367-7108-42)
- Brilliant Violet 421 (#404-7108-42)
- Brilliant Violet 480 (#414-7108-42)
- Brilliant Violet 711 (#407-7108-42)
- eFluor 450 (#48-7108-42)
- eFluor 660 (#50-7108-42)
- Functional Grade (#16-7108-81)
- PE (#12-7108-82)
- PE-Cyanine7 (#25-7108-42)
- PerCP-eFluor 710 (#46-7108-42)
- Request custom conjugation
Product Specific Information
Description:
The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).
Applications Reported:
The JES3-9D7 antibody has been reported for use in capture of human IL-10 by ELISA, intracellular flow cytometric analysis, and for neutralizing IL-10 bioactivity.
(Use Functional Grade purified for functional studies, Product #16-7108-81).
Applications Tested:
The JES3-9D7 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-10 in combination with the biotin JES3-12G8 (Product #13-7109-81) antibody for detection and recombinant human IL-10 (14-8109) as the standard.
A suitable range of concentrations for ELISA capture is 1–4 µg/mL.
A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL–8 pg/mL should be included in each ELISA plate.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-10 encodes a cytokine primarily produced by monocytes, with some production by lymphocytes. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It promotes B cell survival, proliferation, and antibody production. IL-10 also inhibits NF-kappa B activity and participates in JAK-STAT signaling regulation. Knockout studies in mice indicate IL-10 is essential for intestinal immune regulation. Mutations in IL10 are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Associated diseases include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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