Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Alexa Fluor 488 (#53-7108-42)
• Brilliant UV 395 (#363-7108-42)
• Brilliant UV 737 (#367-7108-42)
• Brilliant Violet 421 (#404-7108-42)
• Brilliant Violet 480 (#414-7108-42)
• Brilliant Violet 711 (#407-7108-42)
• eFluor 450 (#48-7108-42)
• eFluor 660 (#50-7108-42)
• Functional Grade (#16-7108-81)
• PE (#12-7108-82)
• PE-Cyanine7 (#25-7108-42)
• PerCP-eFluor 710 (#46-7108-42)
• Request custom conjugation

Product Specific Information

Description:
The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported:
The JES3-9D7 antibody has been reported for use in capture of human IL-10 by ELISA, intracellular flow cytometric analysis, and for neutralizing IL-10 bioactivity.
(Use Functional Grade purified for functional studies, Product #16-7108-81).

Applications Tested:
The JES3-9D7 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-10 in combination with the biotin JES3-12G8 (Product #13-7109-81) antibody for detection and recombinant human IL-10 (14-8109) as the standard.
A suitable range of concentrations for ELISA capture is 1–4 µg/mL.
A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL–8 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-10 encodes a cytokine primarily produced by monocytes, with some production by lymphocytes. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It promotes B cell survival, proliferation, and antibody production. IL-10 also inhibits NF-kappa B activity and participates in JAK-STAT signaling regulation. Knockout studies in mice indicate IL-10 is essential for intestinal immune regulation. Mutations in IL10 are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Associated diseases include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.