Thermo Fisher Scientific IL-12/IL-23 p40 Monoclonal Antibody (C8.3), eBioscience

Applications

Tested Dilution

Publications

ELISA (ELISA)

1-4 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C8.3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468446

Product Specific Information

Description: The C8.3 antibody reacts with the p40 monomer of human IL-12 and IL-23; the antibody preferentially binds to monomeric p40.

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-gamma and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. The p40 and p35 subunits by themselves have no IL-12 bioactivity, though the p40 homodimer has been shown to bind the IL-12 receptor and to be an antagonist of IL-12 p70. Free p40 is typically secreted in vast excess of IL-12 p70 by cells co-expressing both the p35 and p40 subunits. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the IL-6 receptor, while the p35 subunit shows some homology to IL-6 and G-CSF.

Applications Reported: The C8.3 antibody has been reported for use in ELISA.

Applications Tested: The IL-12/IL-23 p40 monomer ELISA using clones C8.3 and C8.6 preferentially measures p40 monomer, exhibiting less than 1.0% cross reactivity with IL-12 (p35p40) and IL-23 (p19p40).

For this assay, the (p40 monomer-specific) purified C8.3 antibody (14-7127) is used as the capture antibody in combination with the biotinylated C8.6 antibody (13-7129) for detection and recombinant human IL-12/IL-23 p40 (39-8128) as the standard. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 10,000 pg/mL - 80.0 pg/mL should be included in each ELISA plate. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.