Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), NovaFluor Yellow 755, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), NovaFluor Yellow 755, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test
Publications: -

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Product Specifications

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Additional Formats

• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 700
• APC / APC-eFluor 780
• Biotin
• Brilliant UV 시리즈 (395, 496, 615, 737, 805)
• Brilliant Violet 시리즈 (605, 650)
• eFluor 시리즈 (450, 506)
• NovaFluor Blue / Red / Yellow 시리즈
• PE / PE-Cyanine / PerCP 시리즈
• Super Bright 시리즈 (436, 600, 645, 702, 780)
• Request custom conjugation available

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Product Specific Information

The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development, excluding plasma cells. CD19 forms a multimolecular complex with CD21, CD81, and MHC class II, contributing to BCR signaling and B cell proliferation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometric analysis of human peripheral blood cells.
Use at 5 µL (0.8 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread impacts.

Excitation: 552 nm
Emission: 690 nm
Laser: 561 nm (Yellow)

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Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and certain myeloid leukemia cells. CD19 serves as a functional regulator of B cell proliferation and is expressed in all B cell precursor leukemias. It acts as a coreceptor for the BCR, lowering the threshold for antigen receptor-dependent stimulation and amplifying signaling. Mutations lead to hypogammaglobulinemia, while overexpression results in B cell hyperactivity.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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