Thermo Fisher Scientific MAPK7 Monoclonal Antibody (OTI3D4), TrueMAB
Human MAPK7(ERK5)에 특이적인 Mouse Monoclonal Antibody로, WB, ICC/IF, Flow Cytometry에 사용 가능. Lyophilized 형태로 제공되며 1 mg/mL 농도. 연구용으로 세포 신호전달 및 발달 관련 MAPK7 분석에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3D4 |
| Immunogen | Full-length human recombinant MAPK7 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAPK7 (ERK5) serine/threonine kinase belongs to the MAP kinase family and regulates various cellular processes including proliferation, differentiation, transcription regulation, and development.
Activated by MAP2K5/MEK5, MAPK7 translocates to the nucleus to phosphorylate and activate transcription factors.
Gene deletion in mice causes defective vascular and cardiac development, leading to embryonic lethality.
MAPK7 is critical for endothelial function and blood vessel integrity.
ERK5 is expressed in many adult tissues—abundantly in heart, placenta, lung, kidney, and skeletal muscle—but not in liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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