Thermo Fisher Scientific AMID Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 43-371 of human AIFM2/FSP1/AMID/AMID (NP_1161861) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AMID,uniProtId:Q9BRQ8-1,ncbiNodeId:9606,antigenRange:43-371,antigenLength:373,antigenImageFileName:PA5-88365_AMID_Q9BRQ8-1_Rabbit.svg,antigenImageFileNamePDP:PA5-88365_AMID_Q9BRQ8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.94 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804860

Product Specific Information

Immunogen sequence: KDSFHHNVAA LRASVETGFA KKTFISYSVT FKDNFRQGLV VGIDLKNQMV LLQGGEALPF SHLILATGST GPFPGKFNEV SSQQAAIQAY EDMVRQVQRS RFIVVVGGGS AGVEMAAEIK TEYPEKEVTL IHSQVALADK ELLPSVRQEV KEILLRKGVQ LLLSERVSNL EELPLNEYRE YIKVQTDKGT EVATNLVILC TGIKINSSAY RKAFESRLAS SGALRVNEHL QVEGHSNVYA IGDCADVRTP KMAYLAGLHA NIAVANIVNS VKQRPLQAYK PGALTFLLSM GRNDGVGQIS GFYVGRLMVR LTKSRDLFVS TSWKTMRQS

Positive Samples: HepG2, A-549; Cellular Location: Cytoplasm, Lipid-anchor, Membrane, Mitochondrion outer membrane

Target Information

The p53 tumor suppressor protein induces cell cycle arrest or apoptosis in response to cellular stresses. PRG3 (p53-responsive gene 3), is induced specifically under p53-dependent apoptotic conditions in human colon cancer cells. PRG3 has significant homology to bacterial oxidoreductases and the apoptosis-inducing factor, AIF. Unlike AIF, PRG3 localizes in the cytoplasm and its ectopic expression induces apoptosis. The PRG3 gene is thus a novel p53 target gene in a p53-dependent apoptosis pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.