Thermo Fisher Scientific Phospho-p53 (Ser15) Monoclonal Antibody (C.381.0)
인간 p53 단백질의 Ser15 인산화 형태를 특이적으로 검출하는 단클론 항체입니다. Western blot, ICC/IF, Flow Cytometry에 사용 가능하며 교차 반응이 없습니다. 고순도 친화 크로마토그래피 정제, 액상 형태로 −20°C 보관합니다.
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Thermo Fisher Scientific Phospho-p53 (Ser15) Monoclonal Antibody (C.381.0)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100 | View 1 publication |
| Flow Cytometry (Flow) | 1:1,600 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C.381.0 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pSer15 of human p53 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 765 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10980596 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with p53 phosphorylated at other sites.
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. Phosphorylation at Ser15 is critical for p53 activation and stabilization. p53 plays a role in blocking proliferation of damaged cells and acts as an anticancer agent.
Mutations in p53 are common in human cancers and lead to loss of tumor suppressor activity. Alterations in the TP53 gene occur both as somatic and germline mutations, such as in Li-Fraumeni syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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