Thermo Fisher Scientific Phospho-JNK1/JNK2 (Thr183, Tyr185) Monoclonal Antibody (E.665.10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200

-

Flow Cytometry (Flow)

1:400

-

Immunoprecipitation (IP)

1:250

-

ChIP assay (ChIP)

1-3 µL

-

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat, Yeast

Published species

Human, Pig, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

E.665.10

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pThr183/Tyr185 of human SAPK/JNK if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:JNK1/JNK2,uniProtId:P45983-1,ncbiNodeId:9606,antigenRange:183|185,antigenLength:427,antigenImageFileName:MA5-15228_JNK1JNK2_P45983-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15228_JNK1JNK2_P45983-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:JNK1/JNK2,uniProtId:P45984-1,ncbiNodeId:9606,antigenRange:183|185,antigenLength:424,antigenImageFileName:MA5-15228_JNK1JNK2_P45984-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15228_JNK1JNK2_P45984-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

591 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10979596

Product Specific Information

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

It is not recommended to aliquot this antibody.

Target Information

C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.