Thermo Fisher Scientific Human C-Reactive Protein (CRP) Polyclonal Antibody, HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000 - 1:30,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200 - 1:500

-

Immunocytochemistry (ICC/IF)

1:200 - 1:500

-

ELISA (ELISA)

1:10,000 - 1:100,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 6.8 to 7.4, with 0.2% BSA

Contains

0.05% Pro-Clean 400

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

Goats were immunized with purified human C-reactive Protein (CRP) of serum or plasma origin. Antiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Target Information

CRP (C-reactive protein) is a major cyclic, pentameric acute phase protein compound consisting of five identical, noncovalently bound, nonglycosylated subunits. Eeach subunit is made up of 206 amino acids, and has a molecular weight of 24 kDa. CRP is produced by the liver, and its plasma levels rise dramatically during inflammatory processes occurring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.), plays an important role in innate immunity, and mediates activities associated with pre-immune nonspecific host resistance. Specifically, there is a 100-1000 fold increase of CRP in response to injury, infection or inflammation. CRP is opsonic, an initiator of the classical complement cascade, and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. CRP is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host, and initiates pathogen elimination by interacting with humoral and cellular effector systems in the blood. Patients with elevated basal levels of CRP have been shown to be an increased risk for hypertension and cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.