
Thermo Fisher Scientific GABRA5 Monoclonal Antibody (N415/24), FITC
GABRA5 단백질을 인식하는 FITC 결합 단클론 항체로, WB, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 단백질 G로 정제된 액상 형태로 제공되며 형광 특성은 498/517 nm. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N415/24 |
| Immunogen | Fusion protein amino acids 368–419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 9.1 mM sodium bicarbonate/PBS (pH 7.4), with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932200 |
Available Formats
- Unconjugated (MA5-27700)
- APC (MA5-45745)
- PE (MA5-45748)
- PerCP (MA5-45747)
- Custom conjugation available upon request
Product Specific Information
A 1:100 dilution of MA5-45746 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 55 kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
This antibody was formerly sold as clone S415-24.
Target Information
GABRA5 is part of GABA, a major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor.
At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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