Thermo Fisher Scientific GABRA5 Monoclonal Antibody (N415/24), APC
GABRA5 단백질을 인식하는 마우스 단클론 항체로, APC로 표지되어 세포 분석에 적합합니다. Western blot, IHC, ICC 등 다양한 응용에 사용 가능하며, 사람, 마우스, 랫트 시료에 반응합니다. 단백질 G로 정제되었으며, 4°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N415/24 |
| Immunogen | Fusion protein amino acids 368–419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5 |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932199 |
Additional Formats
- Unconjugated (MA5-27700)
- FITC (MA5-45746)
- PE (MA5-45748)
- PerCP (MA5-45747)
- Custom conjugation available upon request
Product Specific Information
A 1:100 dilution of MA5-45745 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 55 kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
This antibody was formerly sold as clone S415-24.
Target Information
GABRA5 is a component of the GABA-A receptor complex, a ligand-gated chloride channel mediating inhibitory neurotransmission in the mammalian brain. Chloride conductance can be modulated by agents such as benzodiazepines that bind to the receptor. At least 16 distinct subunits of GABA-A receptors have been identified, and transcript variants utilizing three different alternative non-coding first exons have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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