Thermo Fisher Scientific GABRA5 Monoclonal Antibody (N415/24), PE
GABA A 수용체 알파5 서브유닛을 인식하는 단클론 항체. PE 형광표지로 유세포분석 및 면역형광에 적합. 인체, 마우스, 랫트 반응성. 단백질 G 정제, 보존제 무첨가, 4°C 보관. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N415/24 |
| Immunogen | Fusion protein amino acids 368–419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932202 |
Available Formats
- Unconjugated (MA5-27700)
- APC (MA5-45745)
- FITC (MA5-45746)
- PerCP (MA5-45747)
- Custom conjugation available upon request
Product Specific Information
A 1:100 dilution of MA5-45748 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 55 kDa.
Does not cross-react with GABA A Receptor Alpha2 or other GABA-A receptors based on KO validation data.
This antibody was formerly sold as clone S415-24.
Target Information
GABRA5 is a component of GABA, a major inhibitory neurotransmitter in the mammalian brain acting at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified, and transcript variants utilizing three different alternative non-coding first exons have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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