Thermo Fisher Scientific FOXO4 Monoclonal Antibody (OTI10F12), TrueMAB

Thermo Fisher Scientific FOXO4 Monoclonal Antibody (OTI10F12), TrueMAB

Applications

• Western Blot (WB)

Tested Dilution: 1:2,000

Species Reactivity

• Human
• Mouse
• Rat

Host / Isotype

• Mouse / IgG1

Class

• Monoclonal

Type

• Antibody

Clone

• OTI10F12

Immunogen

Human recombinant protein fragment corresponding to amino acids 206–505 of human FOXO4 produced in E. coli.

Conjugate

• Unconjugated

Form

• Lyophilized

Concentration

• 1 mg/mL

Purification

• Affinity chromatography

Storage Buffer

• PBS, pH 7.3, with 8% trehalose

Contains

• No preservative

Storage Conditions

• −20°C, Avoid freeze/thaw cycles

Shipping Conditions

• Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

FOXO4 (Forkhead box O4) is encoded by the FOXO4 gene located on the X chromosome. Also known as AFX1 or MLLT7, it belongs to the O class of forkhead/winged helix transcription factors (FOXO1, FOXO3, FOXO6).
FOXO4 is abundantly expressed in skeletal muscle and adipose tissue. It binds to the Insulin Responsive Element (IRE) and activates downstream targets of Insulin/IGF1 signaling, such as IGFBP1.
Phosphorylation of FOXO4 by Akt leads to its binding to 14-3-3 and subsequent nuclear export.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.