Thermo Fisher Scientific GTF2B Monoclonal Antibody (OTI5B6), TrueMAB
GTF2B 단백질을 인식하는 Mouse 단클론 항체로 Western Blot에 적합합니다. Human, Mouse, Rat 시료에 반응하며, Lyophilized 형태로 제공됩니다. PBS buffer(pH 7.3, 8% trehalose)로 안정화되어 있으며, -20°C에서 보관합니다. 연구용 전용 제품입니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5B6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 43–316 of human GTF2B produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Ubiquitin is a 76 amino acid, highly conserved eukaryotic polypeptide that marks cellular proteins for proteolytic degradation by the 26S proteasome. This process regulates the concentration and lifespan of key regulatory proteins.
Polyubiquitination involves several enzymatic steps:
- Activation of ubiquitin by E1 (ATP-dependent thioester bond formation)
- Transfer to E2 (ubiquitin-conjugating enzyme)
- Covalent linkage to target proteins directly or via E3 ligase
Deubiquitination enzymes can reverse this modification.
Ubiquitination is involved in numerous cellular processes including:
- Cell cycle regulation
- Growth and differentiation
- Embryogenesis
- Apoptosis
- Signal transduction
- DNA repair and replication
- Stress and immune responses
- Nervous system functions
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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