Thermo Fisher Scientific PTPRC Monoclonal Antibody (OTI2F1), TrueMAB
인간 CD45 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, Flow Cytometry에 적합. 고순도 친화 크로마토그래피 정제, 무보존제, 동결건조 형태. 연구용으로 세포 신호 조절 및 면역학 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:8000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F1 |
| Immunogen | Full-length human recombinant protein of PTPRC (CD45) produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, constituting about 10% of lymphocyte surface proteins. It is absent in non-hematopoietic tissues.
CD45 regulates Src-family kinases and plays a key role in lymphocyte development, antigen signaling, and cytokine receptor regulation through JAK kinase suppression.
Multiple CD45 isoforms arise from alternative splicing, differing in extracellular domains but sharing transmembrane and cytoplasmic regions.
CD45 is also involved in integrin-mediated signaling, apoptosis regulation, and immune function modulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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