Thermo Fisher Scientific CD3 Monoclonal Antibody (OTI3E10), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The CD3 subunit complex is crucial for transducing antigen-recognition signals into T cell cytoplasm and regulating TCR complex expression.
T cell activation involves CD3 gamma, delta, epsilon, and zeta subunits, which are structurally related immunoglobulin superfamily members encoded on human chromosome 11.
These subunits contain cytoplasmic tails with tyrosine-based motifs that mediate signal transduction.
CD3 is expressed on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells.
Decreased T lymphocyte percentages may occur in autoimmune diseases.
Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.