
Thermo Fisher Scientific GFP Monoclonal Antibody (C163)
GFP 단백질을 특이적으로 인식하는 마우스 단클론 항체(C163)로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. 0.5 mg/mL 농도의 액상 형태로 제공되며, Protein A로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 43 publications |
| Immunohistochemistry (IHC) | – | View 6 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | – | View 12 publications |
| Flow Cytometry (Flow) | – | View 1 publication |
| Immunoprecipitation (IP) | – | View 7 publications |
| ChIP assay (ChIP) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C163 |
| Immunogen | GFP isolated directly from the jellyfish Aequorea victoria |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533111 |
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP can be used to localize proteins, study their movement, and research the dynamics of subcellular compartments. GFP technology has provided significant insights into the physiological activities of living cells.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (max. 395 nm) and emits green light (peak emission 508 nm) without requiring additional proteins, substrates, or cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications, including monitoring gene expression, protein localization, protein–protein interactions (yeast two-hybrid), and FRET experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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