Thermo Fisher Scientific Phospho-STAT1 (Tyr701) Monoclonal Antibody (ST1P-11A5)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 20 publications 20 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

ELISA (ELISA)

0.1-1.0 µg/mL

-

ChIP assay (ChIP)

5 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

ST1P-11A5

Immunogen

Synthetic phospho peptide encompassing the conserved C-terminal site (Y701) of murine STAT1 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:STAT1,uniProtId:P42225-1,ncbiNodeId:10090,antigenRange:701,antigenLength:749,antigenImageFileName:33-3400_STAT1_P42225-1_House_mouse.svg,antigenImageFileNamePDP:33-3400_STAT1_P42225-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533113

Product Specific Information

This antibody reacts specifically with the tyrosine-701 phosphorylated form of STAT1 and does not exhibit appreciable cross-reactivity with corresponding tyrosine phosphorylated forms of other STAT proteins or with other endogenous phosphotyrosine-containing proteins. To confirm the exclusive recognition of tyrosine phosphorylated STAT1, Western blots were carried out on lysates of 293 cells transfected with a STAT1 expression vector together with a wild type or kinase ″dead″ JAK1 expression vector. In addition, recognition of endogenous tyrosine phosphorylated STAT1 was confirmed on Western blots with cell lysates derived from serum starved or EGF-stimulated A431 cells and on Western blots with IFNa-stimulated mouse embryo fibroblast lysates.

Target Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.