Thermo Fisher Scientific HSP70 Monoclonal Antibody (MB-H1)
HSP70 단백질을 인식하는 MB-H1 클론의 mouse monoclonal antibody로, Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 0.5 mg/mL 농도의 액상형 비결합 항체로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 4 publications |
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:200 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
| ELISA | Assay-dependent | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MB-H1 |
| Immunogen | This antibody was prepared against full-length human hsp70 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533116 |
Target Information
HSP70 is a heat shock protein (HSP) expressed in response to various biological stresses, including high temperatures. Major HSP families include HSP70, HSP90, and HSP100. The HSP70 family consists of highly conserved proteins induced by stress in all examined organisms.
Human HSP70 family members include:
- HSP70: Strongly inducible and constitutively expressed in primate cells
- HSP72: Induced exclusively under stress conditions
- HSC70: Constitutively expressed; involved in uncoating clathrin-coated vesicles
- GRP78 (BiP): Glucose-regulated 78 kDa protein localized in the endoplasmic reticulum
- HSP75 (p75): Found within mitochondria
HSP70 is encoded by an intronless gene and works with other heat shock proteins to stabilize existing proteins against aggregation and assist in folding newly translated proteins. It also participates in the ubiquitin-proteasome pathway via interaction with AU-rich element RNA-binding protein 1. The HSP70 gene is located in the MHC class III region, clustered with two related genes encoding similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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