Thermo Fisher Scientific P-Selectin (CD62P) Polyclonal Antibody
Thermo Fisher의 P-Selectin (CD62P) 폴리클로날 항체는 인간 반응성을 가지며 다양한 면역학적 분석(WB, IHC, ICC, Flow, ELISA)에 적합합니다. E.coli 유래 재조합 단백질을 면역원으로 사용하였으며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 0.5–1 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL | - |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells | - |
| ELISA | 0.1–0.5 µg/mL | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived human CD62P recombinant protein (Position: W42–G271) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2747087 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type I transmembrane glycoprotein that regulates cell rolling. It is stored in Weibel-Palade bodies of endothelial cells and α-granules of platelets and can be rapidly translocated to the cell surface upon activation by thrombin, histamine, complement 5a, Ca²⁺ ionophores, or ADP.
P-selectin mediates interactions between activated endothelial cells or platelets and leukocytes, functioning as a calcium-dependent receptor binding sialylated Lewis carbohydrate antigens on neutrophils and monocytes. It plays a critical role in inflammation, atherogenesis, thrombosis, and tissue destruction.
Clinically, P-selectin helps distinguish heparin-induced thrombocytopenia with and without thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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