Thermo Fisher Scientific CD171 (L1CAM) Recombinant Mouse Monoclonal Antibody (L1-14.10)
CD171(L1CAM) 인간 ectodomain을 특이적으로 인식하는 재조합 마우스 단클론 항체. WB, IHC, ICC/IF, Flow, IP 등 다양한 응용에 적합. Protein A 정제 및 PBS buffer로 제공되며, 장기 보관 시 -20°C 권장. 신경계 발달 및 종양 연구용으로 최적화.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.003–0.03 µg/mL |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | L1-14.10 |
| Immunogen | Recombinant L1-Fc fusion protein containing the ectodomain of human L1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074685 |
Product Specific Information
This antibody is specific for the ectodomain (amino acids M1–E1123) of human L1 and recognizes an epitope in the third Ig domain.
L1 is overexpressed in various tumors, including neuroblastomas, renal carcinomas, ovarian and endometrial carcinomas, and melanomas.
This antibody does not cross-react with human CHL1.
Target Information
L1CAM/CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family.
The ectodomain consists of multiple immunoglobulin-like domains and fibronectin-like type III repeats, linked via a single transmembrane sequence to a conserved cytoplasmic domain.
This cell adhesion molecule plays a key role in nervous system development, including neuronal migration and differentiation.
Mutations in the L1CAM gene cause X-linked neurological syndromes collectively known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia, and hydrocephalus).
Alternative splicing of a neuron-specific exon is thought to be functionally relevant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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