Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet 786, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue 610-70S, 660-120S
• NovaFluor Red 710, Yellow 610, Yellow 660
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8α/β heterodimer or CD8α/α homodimer. CD8a is found on thymocytes, mature T cells, and NK cells, binding MHC class I and associating with protein tyrosine kinase p56lck to regulate T-cell development and activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.5 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.

Brilliant Violet™ 786 (BV786):

• Tandem dye emitting at 786 nm
• Compatible with violet (405 nm) laser
• Protect from light (photo-oxidation sensitive)
• Not compatible with methanol-based fixation

Recommended Buffers:
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) with minimal impact on brightness and FRET efficiency.

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin family, critical for T-cell signal transduction, differentiation, and activation. CD8 acts as a co-receptor for MHC class I molecules, facilitating cytotoxic T lymphocyte (CTL) activation and pathogen or tumor cell clearance. Expressed on T cells, NK cells, monocytes, and dendritic cells as CD8α/β heterodimer or CD8α homodimer.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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