Thermo Fisher Scientific CD4 Monoclonal Antibody (OKT4 (OKT-4)), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (OKT4 (OKT-4)), Brilliant Violet 786

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 661, Brilliant Violet 650
• eFluor 450, eFluor 660
• FITC
• Functional Grade
• NovaFluor Blue 610-70S, 660-120S, 690
• NovaFluor Yellow 660
• PE, PE-Cyanine7, PerCP-Cyanine5.5
• Super Bright 436, 600, 702
• Request custom conjugation available

Product Specific Information

The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by thymocytes, T-helper cells, and monocytes. CD4 acts as a receptor for HIV.
OKT4 and RPA-T4 recognize different epitopes and do not cross-block each other.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for flow cytometry using human peripheral blood cells
  • Recommended use: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Fluorophore Information:
Brilliant Violet™ 786 (BV786) is a tandem dye emitting at 786 nm, suitable for cytometers with a 405 nm violet laser.
Use Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize non-specific polymer interactions when using multiple polymer dyes.

Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and samples from light.

Fixation:
Samples may be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C in the dark. BV786 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen.

Target Information

CD4 antigen participates in MHC class II recognition and serves as a co-receptor for HIV. It is primarily expressed on T-helper lymphocytes, but also on monocytes, macrophages, and dendritic cells.
Detected in thymus, lymph nodes, tonsils, spleen, and some non-lymphoid tissues such as brain and gut.
CD4 aids in early T-cell activation via interaction with the T-cell receptor complex and Lck kinase. It may mediate neuronal damage in immune-related CNS diseases.
Multiple alternatively spliced transcripts identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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