Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Violet 786, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 421–711 series
• eFluor 450, 506, 660
• FITC
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780 series
• NovaFluor Blue and Yellow series
• Functional Grade
• Request custom conjugation

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b forms a heterodimer with CD18 (Mac-1, αMβ2 integrin) and binds CD54 (ICAM-1), C3bi, and fibrinogen.
Mac-1 is expressed on macrophages, NK cells, granulocytes, activated lymphocytes, and peritoneal B-1 cells. M1/70 also cross-reacts with human CD11b, detecting this antigen on monocytes, granulocytes, and NK subsets.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤ 0.25 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Optimize antibody dilution empirically for best performance.

Fluorochrome Info: Brilliant Violet™ 786 (BV786) emits at 786 nm, excited by 405 nm laser. Ensure cytometer compatibility.

Buffer Recommendations:
When using multiple Brilliant Violet™, Brilliant UV™, or polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (SB-4401-42) or
• Brilliant Stain Buffer™ (00-4409-75)

Light Sensitivity: Protect vial and stained samples from light.
Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (00-8222-49) or
• 1-step Fix/Lyse Solution (00-5333-54)

BV786 is not compatible with methanol-based fixation.
Excitation: 407 nm Emission: 786 nm Laser: Violet

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule forming a heterodimer with CD18 (integrin beta-2), known as Mac-1 or CR3.
Expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia.
Functions as a receptor for ICAM-1/2/4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, mediating cell adhesion and immune interactions.
Commonly used as a microglial marker in nervous tissue. Expression increases during monocyte maturation and varies by tissue.

Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related SLE susceptibility.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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