Thermo Fisher Scientific CD1d Monoclonal Antibody (51.1), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 1 publication

Product Specifications

Available Formats

• Unconjugated
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 51.1 reacts with human CD1d, a member of the CD1 family related to non-polymorphic MHC Class I-like molecules. CD1d is a conserved transmembrane receptor that can associate with beta-microglobulin or exist independently, suggesting multiple mechanisms for glycolipid antigen presentation to NKT cells. Expression occurs on peripheral B cells, resting monocytes, cortical thymocytes, and intestinal epithelial cells. Elevated expression is observed in HCV-infected livers.

The 51.1 antibody has functional blocking activity, inhibiting interactions between CD1d-transfected cells and NKT cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells
• Recommended Use: 5 µL (0.125 µg) per 100 µL test volume
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD1d forms a heavy chain associated with beta-2 microglobulin on cortical thymocytes. Independent expression also occurs on intestinal epithelial cells. It is the sole group 2 member of the CD1 family of MHC-like glycoproteins. The CD1d gene encodes a transmembrane glycoprotein structurally related to MHC proteins, forming heterodimers with beta-2 microglobulin. CD1 proteins mediate lipid and glycolipid antigen presentation to T cells. The human genome contains five CD1 genes on chromosome 1, differing in localization and lipid ligand specificity. CD1d localizes to late endosomes and lysosomes via a tyrosine-based motif. Diseases associated with CD1D include Mycobacterium malmoense infection and autoimmune disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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