Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), Super Bright 600, eBioscience
CD326 (EpCAM) 단일클론 항체(G8.8)는 마우스 EpCAM을 인식하며, Super Bright 600 형광체로 결합되어 유세포분석에 최적화되어 있습니다. 암세포 및 상피세포 표지에 활용되며, 광민감성이 있어 어둠 속 보관이 필요합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), Super Bright 600, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 5 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 26 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G8.8 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2688098 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 645
- Request custom conjugation
Product Specific Information
The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40, 17-1A antigen, TACSTD1, and CD326.
The immunogen used was the TE-71 thymic epithelial cell line. CD326 is expressed on most epithelial cells and considered a pan-carcinoma antigen. It mediates calcium-independent, homophilic cell-cell adhesion and may act as a growth factor receptor.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on TE-71 cell line at ≤0.25 µg/test. Recommended careful titration for optimal performance.
Super Bright 600 is a tandem dye excited at 405 nm and emits at 600 nm. Recommended bandpass filter: 610/20. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.
Light sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
Excitation: 405 nm
Emission: 600 nm
Laser: Violet
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial cells. It mediates Ca²⁺-independent homotypic adhesion and influences cell cycle, proliferation, and metabolism through c-myc, cyclin A, and E induction.
Ep-CAM overexpression is associated with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors. It distinguishes adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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