Thermo Fisher Scientific CD309 (FLK1) Monoclonal Antibody (Avas12a1), Super Bright 600, eBioscience
Mouse FLK1 (VEGFR2/CD309) 인식하는 Avas12a1 단클론 항체로 Super Bright 600 형광체 결합. Flow cytometry에 최적화되어 있으며 405 nm 레이저로 여기, 600 nm에서 방출. 광민감성이 있어 암조건 보관 필요. 연구용으로만 사용.
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Thermo Fisher Scientific CD309 (FLK1) Monoclonal Antibody (Avas12a1), Super Bright 600, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (PFA fixed) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 3 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Avas12a1 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2688140 |
Additional Formats
Product Specific Information
The Avas12a1 monoclonal antibody reacts with mouse Flk-1 (vascular endothelial growth factor receptor 2, VEGFR2, CD309). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and in some adult tissues.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on bEnd.3 cells at ≤1.0 µg per test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.
Fluorochrome Information:
Super Bright 600 is a tandem dye excited by violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass. Ensure instrument compatibility.
Usage Notes:
- Use Super Bright Complete Staining Buffer (Product # SB-4401) when using multiple Super Bright dye-conjugated antibodies to minimize non-specific polymer interactions.
- Light sensitive: protect vial and samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase that binds vascular endothelial growth factor with high affinity. It is expressed early in development by endothelial cell precursors. FLK1 signaling and trafficking are regulated by multiple factors including Rab GTPase, P2Y purine nucleotide receptor, integrin αVβ3, and T-cell protein tyrosine phosphatase. Mutations are implicated in infantile capillary hemangiomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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