Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), Super Bright 600, eBioscience

Applications and Tested Dilution

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Product Specifications

Additional Available Formats

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• Super Bright 436
• Request custom conjugation

Product Specific Information

Description:
The monoclonal antibody GAP.A3 reacts with the human MHC class I molecule HLA-A3, a member of the HLA-A family. HLA-A3 exists as two subtypes (HLA-A3.1 and HLA-A3.2) differing at amino acid positions 152 and 156. MHC class I molecules are expressed on all nucleated cells and present intracellular peptides to cytotoxic T lymphocytes. HLA-A3 is expressed by approximately 15–25% of cancer patients and is known to bind the tumor-associated antigen hTERT.

Applications Reported:
This antibody is reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.5 µg) per 100 µL test volume. Cell count range: 10⁵–10⁸ cells/test.

Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass. Ensure instrument compatibility.

Staining Buffer Recommendation:
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples may be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal signal loss. Clone-specific performance should be empirically validated.

Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

HLA-A is a class I heavy chain paralogue forming a heterodimer with beta-2 microglobulin. It presents peptides from the endoplasmic reticulum to the immune system and is expressed in nearly all cells. The heavy chain (~45 kDa) gene includes 8 exons encoding the leader peptide, peptide-binding domains (α1, α2), α3 domain, transmembrane region, and cytoplasmic tail.
Polymorphisms in exons 2 and 3 determine peptide binding specificity, critical for transplantation typing (bone marrow, kidney).
HLA and MHC antibodies are key in Immunopeptidomics, aiding neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures.

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