Thermo Fisher Scientific CD33 Monoclonal Antibody (WM53)
CD33 단백질을 인식하는 마우스 단클론 항체(WM53)로, 인간 시료에 반응합니다. Western blot, Flow cytometry, Immunoprecipitation에 사용 가능하며, 단백질 G로 정제된 액상 형태입니다. AML 연구 및 면역표지에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | Assay-dependent | |
| Flow Cytometry (Flow) | 1:10–1:50 | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | WM53 |
| Immunogen | Human AML cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538455 |
Additional Formats
Product Specific Information
- Overnight incubation is recommended.
- The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing.
- Acetone fixation for frozen sections is recommended.
- Suggested positive control for IHC: lymphatic tissue.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
- Overnight incubation with primary antibody is recommended.
Target Information
CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that recruit protein tyrosine phosphatases SHP-1 and SHP-2 and mediate receptor internalization.
CD33 is expressed on myelomonocytic lineage cells and binds sialic acid residues on cell surfaces. It serves as a therapeutic target for acute myeloid leukemia (AML) and is also expressed on granulocyte and macrophage precursors in bone marrow, but not on pluripotent stem cells.
As a marker for peripheral monocytes, CD33 helps distinguish myelogenous leukemia cells from lymphoid or erythroid leukemias.
Diseases associated with CD33 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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