Thermo Fisher Scientific CD27 Monoclonal Antibody (LG.7F9), Biotin, eBioscience
Biotin 결합형 CD27 단클론 항체(LG.7F9)는 인간, 마우스, 랫트 CD27에 반응하며, T세포 및 B세포 상호작용 연구에 적합합니다. Flow cytometry에 최적화되어 있으며, 0.125 µg/test 농도로 사용 가능합니다. Affinity chromatography로 정제된 액상 형태로 4°C 암소 보관 권장.
- 카탈로그번호
- 13-0271-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD27 Monoclonal Antibody (LG.7F9), Biotin, eBioscience
Thermo Fisher Scientific CD27 Monoclonal Antibody (LG.7F9), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | View 17 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LG.7F9 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466405 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV 661
- Brilliant Violet 650
- Brilliant Violet 786
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 is found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and plays an important role in T cell-B cell interaction and effector cell maturation. LG.7F9 cross-reacts with human and rat CD27.
Applications Reported:
The LG.7F9 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤ 0.125 µg per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.
Target Information
CD27 is a 50 kDa member of the TNF receptor superfamily, involved in regulation of cell proliferation and death. Expression of CD27 and its ligand CD70 is largely confined to lymphocytes. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subset of B cells. Activation via TCR-CD3 complex upregulates CD27 and leads to release of soluble CD27 (sCD27), a prognostic marker in B cell malignancies. CD27-binding protein (SIVA) is associated with apoptosis induced by CD27 signaling. CD27 dysfunction is linked to Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.