Thermo Fisher Scientific CD27 Monoclonal Antibody (LG.7F9), Biotin, eBioscience

Thermo Fisher Scientific CD27 Monoclonal Antibody (LG.7F9), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 661
• Brilliant Violet 650
• Brilliant Violet 786
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 is found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and plays an important role in T cell-B cell interaction and effector cell maturation. LG.7F9 cross-reacts with human and rat CD27.

Applications Reported:
The LG.7F9 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤ 0.125 µg per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.

Target Information

CD27 is a 50 kDa member of the TNF receptor superfamily, involved in regulation of cell proliferation and death. Expression of CD27 and its ligand CD70 is largely confined to lymphocytes. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subset of B cells. Activation via TCR-CD3 complex upregulates CD27 and leads to release of soluble CD27 (sCD27), a prognostic marker in B cell malignancies. CD27-binding protein (SIVA) is associated with apoptosis induced by CD27 signaling. CD27 dysfunction is linked to Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.