Thermo Fisher Scientific CDK9 Recombinant Rabbit Monoclonal Antibody (1C4E3)
CDK9 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. 인간, 생쥐, 랫드 반응성. HEK293 세포에서 발현된 IgG 형태로, 고순도 친화 크로마토그래피 정제. 연구용 시약으로 세포 주기 및 전사 조절 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:400 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1C4E3 |
| Immunogen | Synthetic peptide corresponding to amino acids 273–372 of human CDK9 (P50750) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.23 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS (pH 7.3) with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911783 |
Product Specific Information
Positive test controls include HeLa, 293T, MCF7, Mouse brain, Rat testis, Rat brain.
The target is typically localized in the cytoplasm, nucleus, and PML body.
Immunogen sequence:
RKVKDRLKAY VRDPYALDLI DKLLVLDPAQ RIDSDDALNH DFFWSDPMPS DLKGMLSTHL TSMFEYLAPP RRKGSQITQQ STNQSRNPAT TNQTEFERVF
Target Information
CDK9 is a serine/threonine kinase that forms an active holoenzyme complex with CyclinT1, playing a key role in regulating the eukaryotic cell cycle. It regulates cytokine-inducible transcription networks and facilitates promoter recognition of transcription factors. CDK9 promotes RNA synthesis in cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also phosphorylates the CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 can lead to cardiac hypertrophy and predisposition to heart failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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