Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), eFluor 450, eBioscience

Applications

Immunohistochemistry (IHC)

• Tested Dilution: N/A
  View 19 publications

Immunocytochemistry (ICC/IF)

• Tested Dilution: N/A
  View 3 publications

Flow Cytometry (Flow)

• Tested Dilution: 0.125 µg/test
  View 163 publications

Immunoprecipitation (IP)

• Tested Dilution: N/A
  View 2 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 506
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780

Product Specific Information

The M5/114.15.2 monoclonal antibody reacts with mouse major histocompatibility complex (MHC) class II glycoproteins encoded by I-A and I-E subregions (I-A^b, I-A^d, I-A^q, I-E^d, I-E^k).
It detects polymorphic determinants on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice with H-2^b, H-2^d, H-2^q, H-2^p, H-2^r, and H-2^u haplotypes, but not H-2^s or H-2^f.

This antibody inhibits I-A-restricted T cell responses of H-2^b, H-2^d, H-2^q, H-2^u haplotypes.
It is validated for flow cytometric analysis of mouse splenocytes and can be used at ≤0.125 µg per test (100 µL final volume).
Cell number should be empirically determined (10⁵–10⁸ cells/test).
Careful titration is recommended for optimal assay performance.

eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm (Violet laser).
Ensure instrument compatibility for detection.
Post-manufacturing filtration: 0.2 µm.

Target Information

MHC class II molecules are transmembrane glycoproteins expressed on antigen-presenting cells (macrophages, dendritic cells, B cells).
They bind endocytosed exogenous antigens and present them to T cells.
The antigen-binding groove between MHC class II α and β chains is open at both ends and accommodates 15–24 amino acid residues.
HLA and MHC antibodies are essential in immunopeptidomics for neoantigen identification and characterization via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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