Thermo Fisher Scientific SARS-CoV/SARS-CoV-2 Spike Protein S1 Chimeric Recombinant Human Monoclonal Antibody (CR3022)
SARS-CoV 및 SARS-CoV-2 Spike Protein S1 도메인에 결합하는 인간 유래 재조합 단일클론 항체. 다양한 면역분석(ICC, ELISA, SPR 등)에 사용 가능. HEK293 세포에서 발현된 IgM 카파형 항체로, 고순도 친화성 크로마토그래피로 정제됨. 연구용으로만 사용.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: Assay-dependent
ELISA
- Tested Dilution: Assay-dependent
Neutralization (Neu)
- Tested Dilution: Assay-dependent
In vitro Assay (IV)
- Tested Dilution: Assay-dependent
Surface Plasmon Resonance (SPR)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Human / IgM, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | CR3022 |
| Immunogen | The original monoclonal antibody was generated through an scFv library derived from peripheral blood lymphocytes of a patient exposed to SARS-CoV. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
This antibody binds the amino acids 318–510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein.
It also binds to P462L-substituted S318–510 fragments of the SARS spike protein.
Target Information
The SARS-CoV-2 virus, responsible for the COVID-19 pandemic, has a spike protein composed of two subunits, S1 and S2.
The S1 subunit binds to the human ACE2 receptor, initiating viral entry and host cell recognition.
SARS-CoV spike protein S1, active during the 2002–2003 outbreak, shares high similarity with SARS-CoV-2 S1, though amino acid differences may affect ACE2 binding affinity.
Both spike proteins have a trimeric structure, with each subunit consisting of two domains:
- N-terminal domain (NTD) – involved in recognition of other host factors
- Receptor-binding domain (RBD) – binds to the ACE2 receptor
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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