Thermo Fisher Scientific SARS-CoV/SARS-CoV-2 Spike Protein S1 Chimeric Recombinant Human Monoclonal Antibody (CR3022)
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SARS-CoV 및 SARS-CoV-2 스파이크 단백질 S1 도메인에 결합하는 인간 재조합 단일클론 항체. 다양한 in vitro 분석 및 ELISA, SPR 등에 사용 가능. HEK293 세포 발현, IgA1 아이소타입, 비결합형 액상 형태로 제공. 연구용으로만 사용.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: Assay-dependent
ELISA
- Tested Dilution: Assay-dependent
Neutralization (Neu)
- Tested Dilution: Assay-dependent
In vitro Assay (IV)
- Tested Dilution: Assay-dependent
Surface Plasmon Resonance (SPR)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Human / IgA1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | CR3022 |
| Immunogen | The original monoclonal antibody was generated through an scFv library derived from peripheral blood lymphocytes of a patient exposed to SARS-CoV. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
This antibody binds the amino acids 318–510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. It also binds to P462L-substituted S318–510 fragments of the SARS spike protein.
Target Information
The SARS-CoV-2 virus, responsible for the COVID-19 pandemic, has a spike protein composed of two subunits, S1 and S2.
- The S1 subunit binds to the human ACE2 receptor, initiating viral entry and host cell recognition.
- The SARS-CoV S1 protein, which mediated ACE2 binding during the 2002–2003 SARS outbreak, shares high similarity with SARS-CoV-2 S1, though sequence differences may affect binding affinity.
- Both spike proteins have a trimeric structure, with each subunit containing two domains: the N-terminal domain (NTD) and the receptor-binding domain (RBD).
- The RBD binds directly to the ACE2 receptor, while the NTD participates in recognition of other host factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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