Thermo Fisher Scientific SARS-CoV/SARS-CoV-2 Spike Protein S1 Chimeric Recombinant Mouse Monoclonal Antibody (CR3022)
SARS-CoV 및 SARS-CoV-2 스파이크 단백질 S1 도메인을 인식하는 재조합 마우스 단일클론 항체입니다. 다양한 면역학적 분석에 사용 가능하며, HEK293 세포에서 발현되었습니다. Protein A로 정제되었으며, 연구용으로만 사용됩니다.
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Applications
- Immunocytochemistry (ICC/IF): Assay-dependent
- ELISA: Assay-dependent
- Neutralization (Neu): Assay-dependent
- In vitro Assay (IV): Assay-dependent
- Surface Plasmon Resonance (SPR): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Mouse / IgG2b, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | CR3022 |
| Immunogen | The original monoclonal antibody was generated through an scFv library derived from peripheral blood lymphocytes of a patient exposed to SARS-CoV. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
This antibody binds to amino acids 318–510 in the S1 domain of the SARS-CoV spike protein as well as the SARS-CoV-2 (COVID-19) spike protein. It also recognizes P462L-substituted S318–510 fragments of the SARS spike protein.
Target Information
The SARS-CoV-2 virus, responsible for the COVID-19 pandemic, has a spike protein composed of two subunits, S1 and S2.
- The S1 subunit binds to the human ACE2 receptor, initiating viral entry and host cell recognition.
- The SARS-CoV S1 protein from the 2002–2003 outbreak shares high similarity with SARS-CoV-2 S1, though amino acid differences may affect ACE2 binding affinity.
- Both spike proteins form trimeric structures, each subunit containing two domains: the N-terminal domain (NTD) and the receptor-binding domain (RBD).
- The RBD binds to the ACE2 receptor, while the NTD is involved in recognition of other host factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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