Thermo Fisher Scientific Phospho-AANAT (Thr29) Polyclonal Antibody

Thermo Fisher Scientific Phospho-AANAT (Thr29) Polyclonal Antibody

Applications

• Western Blot (WB): 0.5 µg/mL

Product Specifications

Product Specific Information

PA1-761 detects phospho serotonin N-acetyltransferase (AA-NAT) from rat tissues.
PA1-761 has been successfully used in Western blot procedures.
By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract.

The immunizing phosphopeptide (Cat. # PEP-064) corresponds to amino-terminal amino acid residues 24–33 of mouse AA-NAT.
Total anti-amino-terminal AA-NAT-specific IgG (Cat. # PA1-760) was affinity purified on resin-coupled non-phosphopeptide (Cat. # PEP-063).
Phospho-specific amino-terminal IgG (Cat. # PA1-761) was subsequently isolated on resin-coupled phosphopeptide (Cat. # PEP-064).
This sequence is completely conserved between mouse, rat, and human AA-NAT protein.

Target Information

Arylalkylamine N-Acetyltransferase (AANAT) is the penultimate enzyme in melatonin synthesis.
Melatonin plays a key role in sleep and circadian rhythm regulation.
Large changes in AANAT abundance are responsible for day/night rhythms in melatonin synthesis in the pineal gland and retina.
AANAT activity is mainly regulated by controlling steady-state protein levels, but additional mechanisms may contribute to rapid melatonin production at the onset of darkness in humans.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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