Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (MAR-1), Super Bright 436, eBioscience

Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (MAR-1), Super Bright 436, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Product Specific Information

The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is up-regulated by the presence of IgE. It forms a tetrameric complex with one beta and two gamma subunits containing ITAM motifs, playing a key role in IgE-mediated allergic reactions.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on MC/9 mouse mast cell line at ≤0.25 µg/test (100 µL final volume). Optimal titration recommended.

Super Bright 436 is excited with the violet laser (405 nm) and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

FceR1 (FcεRIα) is a subunit of the high-affinity receptor for IgE. It forms a tetrameric complex (α, β, and two γ subunits). The receptor triggers allergic responses by coupling allergen-bound IgE with mast cells, leading to histamine and mediator release. This mechanism underlies allergic conditions such as hay fever and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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