Thermo Fisher Scientific IgM Monoclonal Antibody (eB121-15F9), Super Bright 436, eBioscience
마우스 IgM의 μ 중쇄를 인식하는 단클론 항체로, 다른 면역글로불린 클래스와 반응하지 않습니다. Super Bright 436 형광체로 결합되어 유세포 분석(Flow Cytometry)에 적합하며, 405 nm 레이저로 여기되어 436 nm에서 방출됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.5 µg/test
- Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eB121-15F9 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2744809 |
Available Formats
Product Specific Information
Description:
The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly during early stages of B lymphopoiesis and on the surface of mature B cells in bone marrow and peripheral tissues. Fluorochrome-conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.
Applications Reported:
This antibody is reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended use ≤ 0.5 µg/test (in 100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test and should be empirically determined. Optimal performance requires titration.
Fluorochrome Information:
Super Bright 436 can be excited with a 405 nm violet laser and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.
Staining Buffer Recommendation:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.
Excitation / Emission / Laser:
- Excitation: 405 nm
- Emission: 436 nm
- Laser: Violet
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Mouse IgM is an immunoglobulin M antibody produced by B cells in response to antigens. It is the first antibody produced during an immune response and is the largest immunoglobulin in serum. Composed of five heavy and five light chains, IgM activates complement and agglutinates antigens. It plays a key role in the innate immune response and is widely used in research applications such as ELISA, Western blotting, and immunohistochemistry.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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