Thermo Fisher Scientific CD93 (AA4.1) Monoclonal Antibody (AA4.1), Super Bright 436, eBioscience

Thermo Fisher Scientific CD93 (AA4.1) Monoclonal Antibody, Super Bright 436

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test
View 6 publications

Product Specifications

Additional Formats

• APC
• Biotin
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The AA4.1 monoclonal antibody reacts with mouse C1qRp, a 130–140 kDa C-type lectin-like type I transmembrane protein.
AA4.1 antigen is expressed on early stages of B cell development in bone marrow, and detectable in adult spleen and fetal liver.
It can be used with other hematopoietic progenitor markers (Thy-1, Sca-1, c-Kit, CD43, CD24) to distinguish subsets of Lymphoid-Committed Progenitors (CLP).
AA4.1 expression correlates with surface IgM and CD23, defining transitional B cell subsets:

• T1 (AA4.1+/CD23-/sIgMhi)
• T2 (AA4.1+/CD23+/sIgMhi)
• T3 (AA4.1+/CD23+/sIgMlo)

AA4.1 is also expressed in cytoplasmic vesicles of endothelial cells, megakaryoblasts, and platelets.
Monoclonal antibodies AA4.1 and 493 recognize different epitopes of the same molecule.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.25 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best results.

Fluorochrome Information:
Super Bright 436 is excited at 405 nm (violet laser) and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent.
For multiple Super Bright conjugates, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.

Laser Info:

• Excitation: 405 nm
• Emission: 436 nm
• Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD93 is a cell-surface glycoprotein and type I membrane protein, originally identified as a myeloid cell-specific marker.
It is involved in intercellular adhesion and apoptotic cell clearance, rather than C1q receptor function.
The cytoplasmic tail interacts with moesin, linking transmembrane proteins to the cytoskeleton and contributing to cytoskeletal remodeling.

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.

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