Thermo Fisher Scientific HMW E.Coli Host Cell Proteins Polyclonal Antibody
E.coli 유래 고분자량 숙주세포 단백질(HMW HCPs)을 인식하는 토끼 폴리클로날 항체로, WB, ELISA, IP 등에 사용 가능. 비접합 액상 형태이며 -20°C에서 보관. 생물의약품 생산 중 불순물 검출용 연구 시약.
- 카탈로그번호
- 100-401-J05
- 판매단위
- pk
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Thermo Fisher Scientific HMW E.Coli Host Cell Proteins Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:1,000–1:2,000 |
| Immunoprecipitation (IP) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture of high molecular weight (HMW) proteins from E.Coli (BL21). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 77.5 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
Target Information
Host Cell Proteins (HCPs) are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance.
HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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