Thermo Fisher Scientific E.coli Combined HCP Polyclonal Antibody
E.coli 유래 호스트 세포 단백질(HCP)을 검출하기 위한 폴리클로날 항체. Western blot, ELISA, IP에 사용 가능. Rabbit IgG 기반 비결합형 항체로 높은 농도(76 mg/mL) 제공. 연구용으로만 사용.
- 카탈로그번호
- 100-401-M61
- 판매단위
- pk
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Thermo Fisher Scientific E.coli Combined HCP Polyclonal Antibody
Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:1,000–1:2,000 |
| Immunoprecipitation (IP) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.coli (BL21). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 76 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
E.coli Combined HCP Antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. The anti-sera reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
Target Information
Host Cell Proteins (HCPs) are process-related impurities derived from a host cell expression system that may remain in trace amounts in a final drug substance.
HCP Antibodies are used to detect impurities in biologically-based therapeutics.
Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis provides more complete insight into the immunocoverage of protein components of the host cell lysate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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