Thermo Fisher Scientific ETS1 Monoclonal Antibody (8A8)
Human ETS1 단백질을 검출하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 분석에 적합. 비결합형 액상 형태로 제공되며, 안정적인 저장 및 높은 특이성 제공. 연구용으로만 사용.
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Thermo Fisher Scientific ETS1 Monoclonal Antibody (8A8)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | – |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | – |
| Flow Cytometry (Flow) | 1:200–1:400 | 1 publication |
| ELISA | 1:10,000 | – |
| ChIP assay (ChIP) | – | 1 publication |
| Functional Assay | – | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8A8 |
| Immunogen | Purified recombinant fragment of human ETS1 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at −20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10978854 |
Product Specific Information
MA5-15609 targets ETS1 in indirect ELISA, FACS, IF, IHC, and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human ETS1 expressed in E. coli.
MA5-15609 detects ETS1, which has a predicted molecular weight of approximately 50.4 kDa.
Target Information
ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); ETS-1; EWSR2; p54) is a 441 amino acid protein and a member of the DNA-binding ETS protein family.
It is a multifunctional protein with an N-terminal pointed domain, a central transactivation domain, and two auto-inhibitory domains flanked by a conserved C-terminal ETS domain.
ETS1 interacts with factors including PEBP2alphaA and p300/CBP, mediating transactivation of Osteopontin, Presenilin-1, Stromelysin, and Collagenase.
Activated by Ras/MAPK signaling, its expression is up-regulated in resting T-cells.
Activity is modulated by AP-1, Sp-1, c-myb, and MafB. Sp100 activates ETS1 in nuclear bodies.
ETS1 interacts with EAP1/Daxx to repress MMP1 and BCL2 transactivation, and with STAT6 to modulate Socs-1 expression induced by IL-4.
It also interacts with GATA3 and positively regulates Tax-1 dependent IL-5 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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