Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (PAb240)

Applications

ELISA (ELISA)

• Tested Dilution: Assay-dependent

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation. Upon activation, p53 induces transcription of genes such as p21waf1, leading to G1 or G2/M arrest.

p53 structure includes:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerization domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is crucial for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation. p53 plays a key role in suppressing proliferation of damaged cells and acts as an anticancer factor. It is also linked to aging, oxidative stress, and cognitive functions such as learning and memory.

In normal cells, p53 is expressed at low levels, but in transformed cell lines, it is highly expressed and contributes to malignancy. Mutations in TP53 disrupt DNA binding and tumor suppressor activity, frequently observed in various human cancers and inherited conditions like Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.